Determination of total and free cholesterol by using cholesterol oxidase from Streptomyces.
نویسندگان
چکیده
منابع مشابه
Cholesterol oxidase-based determination, by continuous-flow analysis, of total and free cholesterol in serum.
We describe a continuous-flow, automated determination of total cholesterol in serum, which is based on enzymatic hydrolysis of cholesterol esters, oxidation of cholesterol by cholesterol oxidase, and colorimetric measurement of liberated perioxide with 4-aminoantipyrine, phenol, and peroxidase. Free cholesterol is determined with the same AutoAnalyzer II manifold and reagents, except that chol...
متن کاملSTREPTOMYCES FRADIAE, A POTENT PRODUCER OF CHOLESTEROL OXIDASE
Thirteen species of Streptomycetes, Arthrobacters and Mycobacterium were studied for the determination of a potential cholesterol oxidase producer. Only Streptomyces fradiae and Streptomyces rishiriences were found to be capable of producing this enzyme. Streptomyces fradiae was selected for the production of cholesterol oxidase and cultured in different media to find optimum media and con...
متن کاملDetermination of free and total cholesterol by direct chloroform extraction.
Since the Liebermann-Burchard reaction was adopted by Grigaut (1) for the quantitative determination of serum total cholesterol, many modified procedures of the original method have been made. The various procedures have differed in extraction method and in the reagents used in the color development. The separation of cholesterol from its esters with digitonin has had limited application becaus...
متن کاملExtracellular Cholesterol Oxidase from Rhodococcus sp.: Isolation and Molecular Characterization
Background: Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical characterizations of produced CHO enzyme were described. Finally, CHO gene was cloned int...
متن کاملCharacterization of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum.
The FAD-containing enzyme cholesterol oxidase catalyzes the oxidation and isomerization of 3beta-hydroxysteroids having a trans double bond at delta5-delta6 of the steroid ring backbone to the corresponding delta4-3-ketosteroid. Two representative enzymes of this family, namely cholesterol oxidase from Streptomyces hygroscopicus (SCO) and the recombinant enzyme from Brevibacterium sterolicum (B...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1977
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.25.1452